Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG.It also reacts with the light chains of other mouse immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Seramun

Seramun作为一家生物制药公司，已经为客户生产各种实验室诊断和研究试剂超过25年。专注于为客户提供用于人类医疗和兽医诊断的优质产品，这些产品在我们位于德国的实验室开发和生产，并通过个性化定制满足各种规模的实验室的要求。

As a leading developer and manufacturer in vitro diagnostics and reagents for enzyme immunoassays especially of ready-to-use substrates, Seramun Diagnostica GmbH is your partner when it comes to meet special requirements, ensuring highest-level precision, keeping development times short and maintaining quality-controlled products in continual availability.

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No.	品牌	货号	名称	规格
1	seramun	A-001-1-B	Anti-human IgA-Biotin	0.5 mg
2	seramun	A-002-1-HRP	Anti-human IgG - HRP	0.5 mg
3	seramun	A-003-1-AP	Anti-human IgM - AP	0.5 mg
4	seramun	A-007-1-HRP	Anti-human α-1-Fetoprotein-HRP	0.5 mg
5	seramun	A-009-1-AP	Anti-human Haemoglobin-AP	0.5 mg
6	seramun	A-010-1-HRP	Anti-human Lysozyme-HRP	0.5 mg
7	seramun	AGX-5-000-0139-0	B. afzelii DbpA	0.1 mg
8	seramun	AGX-5-000-0140-0	B. burgdorferi DbpA	0.1 mg
9	seramun	AGX-5-000-0143-0	B. afzelii OspA	0.1 mg
10	seramun	AGX-5-000-0199-0	B. spielmanii OspC	0.1 mg
11	seramun	E-021	Serazym® Anti-Borrelia lgG	1x 96 wells
12	seramun	E-023	Serazym® Anti-Borrelia lgM	1x 96 wells
13	seramun	E-049	Serazym® Anti-Francisella tularensis GAM	1x 96 wells
14	seramun	E-054	Serazym® Anti-Cardiolipin-ß2-GP-1 IgG/IgM	1x 96 wells
15	seramun	E-089	Serazym® Anti-Phospholipid screen IgG/IgM	1x 96 wells